A Comparative Assessment of CD271 Immunohistochemical Expression in Mesenchymal Stem Cells from Early-Term and Postdate Human Umbilical Cord

Ali Shakir Abed, Haider Abdulrasool Jaafar

Abstract


Background: The CD271 biomarker has been identified with more specificity for the categorization and detection of Mesenchymal Stem Cells (MSCs) in the Human Umbilical cord (HUC). This marker appears to be insufficient for the separation of MSCs from various tissues, like the blood of the umbilical cord (UC), Whartons jelly (WJ), and other regions. Objective of the study was to estimate quantitatively the immunohistochemical (IHC) expression of CD271 of (MSCs) in the early term and postdate of HUC.

Methods: The samples were taken from 50 normal vaginal deliveries. 25 of the UC samples were classified as normal early term labour and 25 as normal postdate labor. The newborn was born between 37 and 38 weeks (6 days) in both early-term HUCs. In a postdate HUC, delivery is granted an extension beyond 40 weeks plus one to 13 days (any time after the estimated due date of delivery). The age range of pregnant women was 18 to 30.The mothers pregnancy and vaginal delivery were normal, and they were singleton newborns. The sections of UC were stained with CD 271 IHC expression.

Results: IHC expression of CD271 in MSCs was significantly higher in the postdate UC (25295.08 6062.32) compared to the early UC (20653.24 5728.09). Because oxidative stress may coexist with the early term that shows decreased MSCs in UC, this is likely because oxidative stress destroyed the cells. The MSCs are distributed throughout the WJ in a jelly-like substance made of several proteoglycan isoforms and collagen fibers.

Conclusion: The IHC expression of CD271 has been the most expressed for MSCs detection. CD271 reveals an adequate method for insulation of the MSCs in UC. In response to oxidative stress, there was vascular endothelium damage to the UC, which led to less abundant MSCs in early term labor.

Keywords

Embryonic stem cell, Human umbilical cord, Wharton jelly, Biomarker p75NTR, Oxidative stress


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References


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DOI: https://doi.org/10.62940/als.v13i3.2994

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